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Seminal malondialdehyde concentration but not glutathione peroxidase activity is negatively correlated with seminal concentration and motility

Hsieh YY, Chang CC, Lin CS - Int. J. Biol. Sci. (2006)

Bottom Line: Seminal GPx activity was measured by oxidation of reduced nicotinamide-adenine dinucleotide.Seminal MDA levels and GPx activities in both groups were compared.GPx activity is non-significantly correlated with the seminal quality.

Affiliation: Department of Obstetrics and Gynecology, China Medical University Hospital, Taichung, Taiwan.

ABSTRACT

Objectives: Reactive oxygen species (ROS) induced lipid peroxidation is associated with sperm function. Malondialdehyde (MDA) concentration and glutathione peroxidase (GPx) activity represent the lipid peroxidation and spermicidal antioxidant, respectively. We aimed to evaluate the relationship of MDA and GPx levels with sperm parameters.

Patients and methods: Specimens were divided into two groups: group 1. normospermia (n=20); group 2. oligoasthenospermia (n=31). Seminal MDA concentration was measured by thiobarbituric acid reaction method. Seminal GPx activity was measured by oxidation of reduced nicotinamide-adenine dinucleotide. Seminal MDA levels and GPx activities in both groups were compared.

Results: MDA concentrations in both groups were significantly different (1.52 +/- 0.75 vs. 2.25 +/- 0.88 nM, p = 0.0021). GPx activities in both groups were non-significantly different (0.48 +/- 0.11 vs. 0.47 +/- 0.12 U/ml). MDA levels were negatively correlated with the sperm motility (MDA = -0.014 x motility + 2.62, p =0.017) and concentration (MDA = -0.0045 x concentration + 2.23, p = 0.0166). GPx activities were positively but non-significantly correlated with the sperm concentration and sperm motility.

Conclusions: Seminal MDA concentrations are negatively correlated with sperm concentration and motility, which might provide a simple and useful tool in predicting sperm parameters. GPx activity is non-significantly correlated with the seminal quality. Roles of seminal MDA upon spermatogenesis merits further surveys.

The correlations of the sperm concentration with (A) malondialdehyde and (B) glutathione peroxidase activities
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Figure 2: The correlations of the sperm concentration with (A) malondialdehyde and (B) glutathione peroxidase activities

Mentions: Sperm concentration is negatively correlated with MDA level. The formula of their relationship was: MDA = -0.0045 x sperm concentration + 2.23 (Figure 2A, p = 0.0166). Sperm concentration is positively but non-statistically related with GPx level. The formula of their relationship was: GPx = 0.0009 x sperm concentration + 0.42 (Figure 2B, p = 0.0696). We noted the negative correlation between the MDA activity and sperm motility. We establish the formula of their relationship: MDA = -0.014 x sperm motility + 2.62 (Figure 3A, p = 0.017). We also noted the positive, but non-significant correlation between the sperm motility and the GPx activity. The formula of their relationship was: GPx = 0.0012 x sperm motility + 0.42 (Figure 3B, p = 0.134).

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Seminal malondialdehyde concentration but not glutathione peroxidase activity is negatively correlated with seminal concentration and motility

Hsieh YY, Chang CC, Lin CS - Int. J. Biol. Sci. (2006)

The correlations of the sperm concentration with (A) malondialdehyde and (B) glutathione peroxidase activities
© Copyright Policy
Figure 2: The correlations of the sperm concentration with (A) malondialdehyde and (B) glutathione peroxidase activities
Mentions: Sperm concentration is negatively correlated with MDA level. The formula of their relationship was: MDA = -0.0045 x sperm concentration + 2.23 (Figure 2A, p = 0.0166). Sperm concentration is positively but non-statistically related with GPx level. The formula of their relationship was: GPx = 0.0009 x sperm concentration + 0.42 (Figure 2B, p = 0.0696). We noted the negative correlation between the MDA activity and sperm motility. We establish the formula of their relationship: MDA = -0.014 x sperm motility + 2.62 (Figure 3A, p = 0.017). We also noted the positive, but non-significant correlation between the sperm motility and the GPx activity. The formula of their relationship was: GPx = 0.0012 x sperm motility + 0.42 (Figure 3B, p = 0.134).

Bottom Line: Seminal GPx activity was measured by oxidation of reduced nicotinamide-adenine dinucleotide.Seminal MDA levels and GPx activities in both groups were compared.GPx activity is non-significantly correlated with the seminal quality.

Affiliation: Department of Obstetrics and Gynecology, China Medical University Hospital, Taichung, Taiwan.

ABSTRACT

Objectives: Reactive oxygen species (ROS) induced lipid peroxidation is associated with sperm function. Malondialdehyde (MDA) concentration and glutathione peroxidase (GPx) activity represent the lipid peroxidation and spermicidal antioxidant, respectively. We aimed to evaluate the relationship of MDA and GPx levels with sperm parameters.

Patients and methods: Specimens were divided into two groups: group 1. normospermia (n=20); group 2. oligoasthenospermia (n=31). Seminal MDA concentration was measured by thiobarbituric acid reaction method. Seminal GPx activity was measured by oxidation of reduced nicotinamide-adenine dinucleotide. Seminal MDA levels and GPx activities in both groups were compared.

Results: MDA concentrations in both groups were significantly different (1.52 +/- 0.75 vs. 2.25 +/- 0.88 nM, p = 0.0021). GPx activities in both groups were non-significantly different (0.48 +/- 0.11 vs. 0.47 +/- 0.12 U/ml). MDA levels were negatively correlated with the sperm motility (MDA = -0.014 x motility + 2.62, p =0.017) and concentration (MDA = -0.0045 x concentration + 2.23, p = 0.0166). GPx activities were positively but non-significantly correlated with the sperm concentration and sperm motility.

Conclusions: Seminal MDA concentrations are negatively correlated with sperm concentration and motility, which might provide a simple and useful tool in predicting sperm parameters. GPx activity is non-significantly correlated with the seminal quality. Roles of seminal MDA upon spermatogenesis merits further surveys.

View Similar Images In: Results  - Collection
View Article: PubMed Central -  PubMed
Show All Figures - Show MeSH
getmorefigures.php?pmc=1457038&rFormat=json&query=null&req=5